关键词: 丝素蛋白, 纤维膜, 茜草素, 静电纺丝, 止血, 抗菌

Abstract: Sudden bleeding accident is one of the important causes of human death, and the death caused by massive bleeding is a considerable medical problem. Therefore, the rapid control of bleeding has become the focus of clinical treatment, and is of important significance in military trauma and traffic accidents. Currently, few antibacterial hemostatic materials developed can simultaneously meet the requirements of low price, rapid hemostatic and low side effects, so there is still huge potential in the development of safe and non-toxic antibacterial hemostatic materials. Silk fibroin is a natural polymer, and has been studied and applied in the field of biomedical materials due to its good hemostatic performance, biocompatibility and biodegradability. However, the antibacterial property of the homogenous silk fibroin protein material is insufficient, which limits its application in wound dressing. In recent years, although inorganic antibacterial agents and organic antibacterial agents are widely used and have high bacteriostatic efficiency, they are still difficult to avoid the environmental problems caused by chemical synthesis and the drawbacks of harming human health. Natural antibacterial agents have become a better choice in the field of medical materials. Alizarin extracted from the medicinal plant madder, is a natural antibacterial agent, has few side effects on the human body, and has hemostatic, anti-inflammatory and other effects.
In this paper, alizarin was used as natural antiseptic and hexafluoroisopropyl alcohol was used as solvent to dissolve silk fibroin protein and prepare a spinning solution. The microscopic morphology, water absorption, hemostatic and antibacterial properties of silk fibroin/alizarin composite fiber membranes were investigated under different proportions.
The results show that, compared with pure silk fibroin fiber membranes, the diameter distribution of the composite fiber membrane with alizarin has no obvious difference. The fibers in the membrane are compact and smooth without beading morphology. At the same time, new absorption peaks appear on the infrared curve of the composite fiber membrane, corresponding to the out-of-plane bending vibration of the C-H bond in the benzene ring, the in-plane bending vibration of C-H plane, and the out-of-plane bending vibration of C-H. The changes of the above peaks indicate the existence of alizarin in the silk fibroin film. Compared with hemostatic gauze, the water absorption of silk fibroin protein film has a significant advantage, and the water absorption of the composite film gradually decreases with the increase of alizarin content. Compared with the BCI value of hemostatic gauze, the BCI value of the composite film decreases gradually with the increase of alizarin content, and the addition of alizarin significantly improves the hemostatic performance of the film. Compared with pure silk fibroin protein membrane, the antibacterial activity of the composite membrane is significantly improved, and the antibacterial rate increases with the increase of the ratio of silk fibroin to alizarin. At the same time, under the condition that the mass ratio of silk fibroin to alizarin of 10:1, the antibacterial rates of the composite membrane are both more than 90% against Staphylococcus aureus and Escherichia coli, showing strong antibacterial effect. Silk fibroin and alizarin are natural substances, with little harm to human body. Silk/alizarin composite membranes have good effect in hemostasis and antibacterial test, and show great application prospect in the development of silk fibroin wounds.

Key words: silk fibroin, fiber membrane, alizarin, electrospinning, hemostasis, antibacterial