Identification and inhibition of microbes on mildewed cotton clothes

doi:10.19398/j.att.202205047

Abstract

Abstract: During the storage or wearing process of cotton clothing, "mildew" phenomenon is caused by microbial contamination, which forms mildew spots on the clothing, produces odor, and affects the appearance and service life of clothing. Wearing moldy clothes is likely to cause cross-infection of the skin and there are health risks. Fungi can decompose fibers to be nutrients, and metabolites secreted by the human body such as sebum and sweat adsorbed on clothing can also provide nutrients for microorganisms to reproduce and grow on them. Molds also secrete organic acids during the growth process to erode the fabric. Cotton clothes in the daily life of the environment is easy to be contaminated by microorganisms, and is more prone to molding in the humid rainy environment. However, there are differences in the types of mold on different fabrics. Therefore, it is necessary to identify the types of mold microorganisms on clothing in different regions, and use effective bacteriostatic agents to protect textiles and prolong their service life.
In order to determine the type of mildew on cotton clothes, so as to inhibit the mildew of cotton clothes, the mold on the moldy clothing was extracted, and was cultured and purified in PDA medium under the appropriate temperature and humidity. Single mold and dominant mixed strains were inoculated on cotton fabrics. In order to simulate the real growth of mold on cotton clothes, human sebum was used as microbial nutrient. Potassium sorbate, zinc sulfate and sodium benzoate were used as antibacterial agents to observe the growth of fungi on cotton fabrics. The number of molds on it was counted by a mold counter to explore the antibacterial effect of the three inhibitors and the optimal addition amount. Finally, seven kinds of molds were obtained. The results showed that the dominant fungi causing mildew of cotton fabrics were Aspergillus and Penicillium. The seven fungi were identified as Rhizopus stolonifera, Penicillium apicale, Aspergillus flavus, Cladosporium budding, Penicillium citrinum, Aspergillus versicolor and Phoma sp. by ITS sequence analysis and homology comparison. It was found that the best bacteriostatic concentrations of different molds were different, and the bacteriostatic effect of zinc sulfate was the best. When the addition of potassium sorbate and zinc sulfate was 0.12g/100mL and the addition of sodium benzoate was 0.14g/100mL, the optimum inhibitory concentration of the mixed dominant fungi on cotton fabrics was obtained.
Identification of mildew fungi on cotton clothes provides experimental reference for elucidating the mechanism of cotton clothes mildew. The optimum concentration of antibacterial agents for cotton fabrics explored lays an experimental foundation for the antibacterial technology of cotton fabrics in the storage end.

Key words: mildew cotton clothing, mold, sequence analysis, antibacterial agent

摘要： 为了确定霉变棉衣物上霉菌的种类,抑制纯棉衣物霉变,对霉变衣物上的霉菌进行提取、分离和纯化,最终得到了7种霉菌。结果发现引起棉织物霉变的优势霉菌菌属为曲霉属霉菌和青霉属霉菌。采用 ITS 序列分析并进行同源性比较,鉴定出这7种霉菌分别为匍枝根霉、顶青霉、黄曲霉、芽枝状枝孢霉、桔青霉、杂色曲霉和茎点霉菌。将单个的优势霉菌接种到棉织物上,采用山梨酸钾、硫酸锌和苯甲酸钠作为抑菌剂,探究其对霉变棉织物的抑制效果。结果表明:不同霉菌最佳抑菌浓度不同,且3种抑菌剂硫酸锌的抑菌效果最好。当山梨酸钾和硫酸锌添加量为0.12 g/100mL,苯甲酸钠添加量为0.14 g/100mL时为棉织物上混合优势霉菌最佳抑菌浓度。本文鉴定了棉衣物上致霉霉菌菌属,并确定了3种抑菌剂最佳抑菌浓度,为开发有效的纺织品防霉技术奠定基础。

关键词: 霉变棉衣物, 霉菌, 序列分析, 抑菌剂

CLC Number:

TS107.4

XIAO Yuyan, REN Zehua, YANG Yu, GAO Yuan, LIU Jianli. Identification and inhibition of microbes on mildewed cotton clothes[J]. Advanced Textile Technology, 2023, 31(1): 240-247.

肖雨嫣, 任泽华, 杨宇, 高源, 刘建立. 霉变棉衣物上微生物的鉴定与抑制[J]. 现代纺织技术, 2023, 31(1): 240-247.

References

[1] 马琰,李永辉,刘志军.室内霉菌污染的环境成因与健康人居[J].新建筑,2019(5):28-31.
MA Yan, LI Yonghui, LIU Zhijun. Environmental causes of indoor mold pollution and healthy living[J]. New Architecture, 2019(5): 28-31.
[2] FAHY W P, KRIFA M, FAJAGANESE S. Perspectives on textile cleanliness:Detecting human sebum residues on worn clothing[J]. Textile Research Journal, 2019, 89(23-24): 5226-5237.
[3] 路智勇,惠任.纺织品文物霉害预防性控制[J].四川文物,2009(3):89-92.
LU Zhiyong, HUI Ren. Preventive control of mildew in textile cultural relics[J]. Sichuan Cultural Relics, 2009(3): 89-92.
[4] 李世超,唐长波,黄阳阳.丝绸纹样档案保护中的霉菌防治[J].档案与建设,2017(3):88-90.
LI Shichao, TANG Changbo, HUANG Yangyang. Mold control in the protection of silk pattern archives[J]. Archives and Construction. 2017(3): 88-90.
[5] 刘俊,刘佳明,周铁丽,等.温州地区皮革制品霉菌分离及自配防霉剂研究[J].中国皮革,2013,42(11):20-24.
LIU Jun, LIU Jiaming, ZHOU Tieli, et al. Study on mold isolation and self-preparation of antifungal agent in leather products in Wenzhou [J]. China Leather, 2013, 42(11): 20-24.
[6] 王丽,张毓,陈翠岚.我国食品防腐剂的应用及发展趋势[J].食品安全质量检测学报,2011,2(2):83-87.
WANG Li, ZHANG Yu, CHEN Cuilan. Application and development trend of food preservatives in my country[J]. Journal of Food Safety and Quality Inspection,2011, 2(2): 83-87.
[7] LÓPEZ O V, GIANNUZZI L, ZARITZKY N E, et al. Potassium sorbate controlled release from corn starch films[J]. Materials Science and Engineering: C, 2013, 33(3): 1583-1591.
[8] FASIHNIA S H, PEIGHAMBARDOUST S H, PEIG-HAMBARDOUST S J, et al. Development of novel active polypropylene basedpackaging films containing different concentrations of sorbic acid [J]. Food Packaging and Shelf Life, 2018, 18: 87-94.
[9] 邢海丽,辛嘉英,王艳,等.响应面优化甲烷氧化菌素、苯甲酸钠和山梨酸钾复配剂抑菌效果[J].食品科学,2016,37(18):1-5.
XING Haili, XIN Jiaying, WANG Yan,et al. Response surface methodology to optimize the antibacterial effect of methanotrophin, sodium benzoate and potassium sorbate compound [J]. Food Science, 2016, 37(18):1-5 .
[10] SUN T, HAO H, HAO W T, et al. Preparation and antibacterial properties of titanium-doped ZnO from different zinc salts[J]. Nanoscale Research Letters, 2014, 9(1):98.
[11] 戴芳澜.中国真菌总汇[M].北京:科学出版社,1979:50-85.
DAI Fanglan. China Fungi CollectionM]. Beijing: Science and Technology Press, 1979:50-85.
[12] 冯长海.改良透明胶带法在丝状真菌形态观察中的应用[J].临床检验杂志,2017,35(10):32-33.
FENG Changhai. Application of modified scotch tape method in morphological observation of filamentous fungi[J]. Journal of Clinical Investigation, 2017, 35(10): 32-33.
[13] HO K J, HEE K Y, RAN L A, et al. Manufacture of Koji using fungi Isolation from Nuruk and identification of Koji molds[J].The Korean Journal of Mycology, 2012,40(4):187-190
[14] 阎淑雅,鄂亚平.正常成人皮脂成分含量的测定[J].中华皮肤科杂志,1992,25(1):32-34.
YAN Shuya, E Yaping. Determination of sebum components in normal adults[J].Chinese Journal of Dermatology, 1992, 25(1): 32-34.
[15] 虞雅伦.皮脂污化纺织品老化黄变性能研究及抗黄变洗护用品开发[D].杭州:浙江理工大学,2016.
YU Yalun. Research on Aging Yellowing Properties of Sebum-Stained Textiles and Development of Anti-Yellowing Toiletries[D]. Hangzhou: Zhejiang Sci-Tech University,
2016.
[16] 叶晓婉,朱润琪,倪新程,等.明党参内生菌的分离鉴定及其对金黄色葡萄球菌的抑制作用[J].微生物学杂志,2019,39(3):35-43.
YE Xiaowan, ZHU Runqi, NI Xincheng,et al. Isolation and identification of endophytic bacteria from Codonopsis pilosula and their inhibitory effect on Staphylococcus aureus[J]. Journal of Microbiology, 2019, 39(3):35-43.